Porcine Endothelin 1 - ET-1 ELISA Kit

Mfr.No.

E0230Po

Sensitivity

1.52ng/ml

Detection Range

3-700ng/ml

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Big endothelin-1;EDN 1;EDN1;Endothelin-1;ET-1;PPET1;Preproendothelin-1

Molecular Weight

23259

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0230Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	103.3	4.54	4.4
Intra-Assay	2	18	216.5	9.85	4.5
Intra-Assay	3	18	340.7	8.26	2.4

Background

Endothelins are endothelium-derived vasoconstrictor Peptides (PubMed: 2451132). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (By similarity).

Protocol

These standard curves of E0230Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400ng/ml	1.959	1.888	1.827
	1.817
200ng/ml	0.89	0.916	0.855
	0.943
100ng/ml	0.443	0.537	0.476
	0.63
50ng/ml	0.167	0.188	0.128
	0.21
25ng/ml	0.146	0.132	0.071
	0.117
0ng/ml	0.061	0.061	0
	0.06

Gene ID	396915
UniProt ID	P09558

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110638	E0230Po	48T	$320		Add to Cart
BHE12110638	E0230Po	96T	$458		Add to Cart

Porcine Endothelin 1 - ET-1 ELISA Kit

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