Porcine Creatine Kinase M-type

Mfr.No.

E0553Po

Alternative Names

CKM;CPK-M;Creatine kinase M chain;Creatine kinase M-type;Creatine phosphokinase M-type;M-CK

Reactivity

Porcine

Detection Range

0.05-20mU/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

43059

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.025mU/mL

Background

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (By similarity).

Gene ID	397264
UniProt ID	Q5XLD3
Antigen	CKM

Research Area

Metabolism

Protocol

These standard curves of E0553Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12mU/mL	2.306	2.171	2.124
	2.036
6mU/mL	1.725	1.625	1.578
	1.524
3mU/mL	1.173	1.092	1.046
	1.011
1.5mU/mL	0.71	0.741	0.695
	0.772
0.75mU/mL	0.492	0.446	0.399
	0.4
0mU/mL	0.043	0.047	0
	0.05

Precision

We measured random samples of E0553Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.6	0.24	5.3
Intra-Assay	2	18	1.4	0.11	7.7
Intra-Assay	3	18	2.8	0.21	7.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110926	E0553Po	48T	$320		Add to Cart
BHE12110926	E0553Po	96T	$458		Add to Cart

Porcine Creatine Kinase M-type - CKM ELISA Kit

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