Porcine Catechol-O-methyltransferase

Mfr.No.

E0445Po

Sensitivity

0.11ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Catechol-O-Methyltransferase (also known as COMT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Catechol O-methyltransferase;COMT;Fragment

Molecular Weight

20587

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E0445Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	13.19	0.57	4.36
Intra-Assay	2	18	18.33	0.59	3.2
Intra-Assay	3	18	4.55	0.2	4.34

Background

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

Protocol

These standard curves of E0445Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.398	2.516	2.471
	2.635
20ng/ml	1.598	1.513	1.468
	1.429
10ng/ml	0.886	0.802	0.757
	0.717
5ng/ml	0.411	0.465	0.42
	0.519
2.5ng/ml	0.194	0.234	0.189
	0.273
0ng/ml	0.049	0.045	0
	0.041

Gene ID	100155530
UniProt ID	Q99028

Research Area	Neuroscience; Signal Transduction; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110834	E0445Po	48T	$320		Add to Cart
BHE12110834	E0445Po	96T	$458		Add to Cart

Porcine Catechol-O-methyltransferase - COMT ELISA Kit

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