POD (peroxidase) Assay kit

Mfr.No.

EU2611

Sensitivity

1.875pg/ml

Detection Range

3.12-200pmol/ml

Reactivity

Universal

Assay Duration

20 minutes

Alternative Names

POD ELISA Kit, peroxidase ELISA Kit

Detection Method

Enzyme catalysis

Recovery

Add a certain amount of POD into the sample. Calculate the recovery by comparing the measured value with the expected amount of POD in the sample.

Sample Type	Recovery Range(%)	Average(%)
serum(n=10)	91-104	99
EDTA plasma(n=10)	90-104	96
Heparin plasma(n=10)	95-105	99

Linearity

Dilute the sample with a certain amount of POD at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type	1:2	1:4	1:8
serum(n=10)	90-98%	88-100%	85-100%
EDTA plasma(n=10)	84-99%	83-99%	82-96%
Heparin plasma(n=10)	83-93%	80-93%	86-100%

Specificity

Specifically binds with POD , no obvious cross reaction with other analogues.

Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(pmol/ml)	OD-1	OD-2	Average
0	0.07	0.066	0.068
3.125	0.275	0.23	0.252
6.25	0.333	0.344	0.338
12.5	0.582	0.609	0.595
25	0.817	0.82	0.818
50	1.412	1.435	1.423
100	1.81	1.892	1.851
200	2.692	2.615	2.653

Sample Type

Tissue lysate, Cell lysates, Cell culture supernatants, Plasma, Other biological fluids

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pmol/ml)	5.88	23.69	102.4	6	23.85	101.41
Standard deviation	0.31	1.21	5.56	0.28	1.49	5.05
CV(%)	5.32	5.12	5.43	4.69	6.23	4.98

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5)	37°C for 1 month	2-8°C for 6 months
Average(%)	80	95-100

Notes

For Research Use Only

Usage Notes

Step 1:
Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37°C.

Washing:
Wash the plate twice without immersion.

Step 2:
Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37°C.

Washing:
Wash the plate five times without immersion.

Step 3:
Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)

Step 4:
Add 50ul stop solution. Read at 450nm immediately and calculate.

Antigen	POD

Applications	ELISA

Storage	2-8°C(Sealed), Don't cryopreserve.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10808344	EU2611	96T	$520		Add to Cart

POD (peroxidase) Assay kit

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