Pig porcine TGF Alpha ELISA Kit PicoKine®

Mfr.No.

EK0511-PO

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Pig

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.102	0.199	0.291	0.443	0.711	1.156	1.676	2.315

Description

Pig porcine TGF Alpha ELISA Kit PicoKine® (96 Tests). Quantitate Pig TGFA in cell culture supeatants, serum, plasma (heparin, EDTA) and Pig porcine milk. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Protransforming growth factor alpha;Transforming growth factor alpha;TGF-alpha;EGF-like TGF;ETGF;TGF type 1;TGFA;

Molecular Weight

17006 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V40-A89

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.

Subcellular Localization

Transforming growth factor alpha: Secreted, extracellular space.

Sample Type

Cell culture supeatants, Serum, Plasma, Pig porcine milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	118	225	516	126	238	509
Standard deviation	5.43	12.83	16.5	8.95	14.28	27
CV (%)	4.6	5.7	3.2	7.1	6	5.3

Protein Function

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Background

Transforming growth factor alpha (TGF-alpha) is upregulated in some Pig porcine cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene (TGFA) maps to Pig porcine chromosome 2 close to the breakpoint of the t (2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic Pig porcine TGF-alpha showed similar immunoreactivity when compared with Pig porcine TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	397484
Gene Name	TGFA
UniProt ID	P01135
Protein Name	Protransforming growth factor alpha
Tissue Specificity	Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. .

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Growth Factors,Growth Factors/Hormones,Invasion/Microenvironment,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001184	EK0511-PO	96 wells/kit	$499		Add to Cart

Pig porcine TGF Alpha ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Pig porcine TGF Alpha ELISA Kit PicoKine®

Rate it now..!!