Pig porcine Epiregulin ELISA Kit PicoKine®

Mfr.No.

EK1394-PO

Sensitivity

<10 pg/ml

Detection Range

3.9 pg/ml - 250 pg/ml

Reactivity

Pig

Sample Volume

Concentration (pg/ml)	0	3.9	7.8	15.6	31.2	62.5	125	250
O.D.	0.063	0.103	0.180	0.301	0.531	0.8779	1.426	2.062

Description

Pig porcine Epiregulin ELISA Kit PicoKine® (96 Tests). Quantitate Pig EREG in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Proepiregulin;Epiregulin;EPR;EREG;

Molecular Weight

19044 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V60-L108

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Subcellular Localization

Epiregulin: Secreted, extracellular space .

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	26.2	88.7	144	25.4	91.5	151
Standard deviation	1.18	7.94	6.81	1.73	7.05	11.93
CV (%)	4.5	5.8	5.7	6.8	7.7	7.9

Protein Function

Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed:9419975). Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357). .

Background

Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	100620969
Gene Name	EREG
UniProt ID	O14944
Protein Name	Proepiregulin
Tissue Specificity	In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon. .

Applications	ELISA
Research Area	Cancer,Growth Factors/Hormones,Signal Transduction,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001191	EK1394-PO	96 wells/kit	$499		Add to Cart

Pig porcine Epiregulin ELISA Kit PicoKine®

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