Pig Immunoglobulin G(IgG) ELISA Kit

Mfr.No.

CSB-E06804p

Alternative Names

Immunoglobulin G(IgG)

Reactivity

Pig

Detection Range

0.586 ug/mL-150 ug/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human FRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	98	95-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Sensitivity

0.467 ug/mL

Limit Of Detection

450 nm

Antigen	Immunoglobulin G(IgG)

Applications

ELISA

Research Area

IgG

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli. Wu S et al,Peptides,2012 Jun;35(2):225-30 Dietary N-Carbamylglutamate Supplementation Boosts Intestinal Mucosal Immunity in Escherichia coli Challenged Piglets Fengrui Zhang et al,PLoS One,2013 Oral administration of N-carbamylglutamate might improve growth performance and intestinal function of suckling piglets Xiangfang Zeng.et al,Livestock Science,181: 242-248 Effects of dietary supplementation with cupreous N-carbamylglutamate (NCG) chelate and copper sulfate on growth performance, serum biochemical profile and immune response, tissue mineral levels and fecal excretion of mineral in weaning piglets Peng Liao.et al,Food and Agricultural Immunology ,Jul 1 Effect of dietary copper source (inorganic vs. chelated) on immune response, mineral status, and fecal mineral excretion in nursery piglets Peng Liao.et al,Food and Agricultural Immunology ,Dec 17 Effects of dietary supplementation with essential oils and organic acids on the growth performance, immune system, faecal volatile fatty acids and microflora community in weaned piglets Yang CM, et al,Journal of Animal Science,November 2 Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance, blood indexes, and intestinal barrier function of weanling piglets Zong X, et al,Livestock Science,December 24

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