Pig Epithelial growth factor,EGF ELISA Kit

Mfr.No.

CSB-E06788p

Sensitivity

125 pg/mL

Detection Range

250 pg/mL-10000 pg/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Epithelial growth factor,EGF

Recovery

The recovery of rat GAD autoantibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-98
EDTA plasma (n=4)	93	87-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GAD autoantibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	86
	Range %	81-97
1:10	Average %	89
	Range %	85-100
1:20	Average %	95
	Range %	90-100
1:40	Average %	93
	Range %	86-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	F1S137

Applications	ELISA
Research Area	EGF

Storage	Store at 2-8°C. Please refer to protocol.

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