Pig Cystatin C,Cys-C ELISA Kit

Pig Cystatin C,Cys-C ELISA Kit

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Mfr.No.

CSB-E13282p

Sensitivity

0.2 ug/mL

Detection Range

0.78 ug/mL-50 ug/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Cystatin C,Cys-C, ARMD11, MGC117328, OTTHUMP00000164181, OTTHUMP00000164182, bA218C14.4 (cystatin C), cystatin 3, gamma-trace, neuroendocrine basic polypeptide, post-gamma-globulin

Recovery

The recovery of human ARL8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-104
EDTA plasma (n=4)	90	85-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ARL8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	96
	Range %	90-100
1:10	Average %	93
	Range %	89-97
1:20	Average %	104
	Range %	99-109
1:40	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.680	2.752	2.716	2.538
500	1.979	2.076	2.028	1.850
250	1.230	1.214	1.222	1.044
125	0.773	0.739	0.756	0.578
62.5	0.455	0.476	0.466	0.288
31.2	0.384	0.369	0.377	0.199
15.6	0.270	0.286	0.278	0.100
0	0.175	0.181	0.178

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q0Z8R0

Applications	ELISA
Research Area	CST3

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Remote Ischemic PreconditioningMay Attenuate Renal Ischemia-Reperfusion Injury in a Porcine Model of Supraceliac Aortic Cross-Clamping Athanasiadis D.et al,J Vasc Res,2015 52:161-171 Impact of high doses of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model Witt L. et al,Paediatr Anaesth,26(3):259-65.

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SKU	Supplier No.	Size	Your price
BHE10507050	CSB-E13282p	96T	$835	Add to Cart
BHE10507050	CSB-E13282p	5x96T	$4008	Add to Cart
BHE10507050	CSB-E13282p	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.