Pig Corticoliberin(CRH) ELISA kit

Mfr.No.

CSB-EL005963PI

Alternative Names

corticotropin releasing hormone, CRF, corticotropin-releasing factor

Reactivity

Pig

Detection Range

100 pg/mL-1600 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of horse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-97
EDTA plasma (n=4)	106	101-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	84
	Range %	80-88
1:4	Average %	95
	Range %	90-100
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Sensitivity

100 pg/mL

Limit Of Detection

450 nm

UniProt ID	P06296
Antigen	Corticotropin Releasing Hormone

Applications

ELISA

Research Area

CRH

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10507040	CSB-EL005963PI	10x96T	$7505	Add to Cart