Pig Anti-SST(Anti-Somatostatin Antibody) ELISA Kit

Mfr.No.

ELK8180

Sensitivity

1.24 ng/mL

Detection Range

3.13-200 ng/mL

Reactivity

Pig

Assay Duration

2.5h

Assay Type

Competitive Inhibition

Alternative Names

GHIH; SRIF; SMST; GHRIH; GHRIF; Growth Hormone Releasing Inhibiting Hormone; Somatotropin Release-Inhibiting Factor; Somatostatin-14; Somatostatin-28

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-SST and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-SST in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	97-105%	101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-SST and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-97%	78-96%	80-99%	89-97%
EDTA plasma(n=5)	93-104%	87-96%	82-95%	78-99%
Heparin plasma(n=5)	95-104%	93-105%	82-101%	91-103%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
200.00	0.191
100.00	0.386
50.00	0.655
25.00	0.878
12.50	1.139
6.25	1.578
3.13	1.796
0.00	2.232

Sample Type

Serum, Plasma, Other biological fluids

Limit Of Detection

200 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig Anti-SST protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Anti-SST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig Anti-SST in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Tumor immunity;Endocrinology;Hormone metabolism;

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15200356	ELK8180	48T	$412		Add to Cart
BHE15200356	ELK8180	96T	$588		Add to Cart

Pig Anti-SST(Anti-Somatostatin Antibody) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Pig Anti-SST(Anti-Somatostatin Antibody) ELISA Kit

Rate it now..!!