Mouse Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®

Mfr.No.

EK2063

Sensitivity

<50 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.038	0.061	0.070	0.106	0.207	0.456	1.035	1.855

Description

Mouse Vitamin DBP ELISA Kit PicoKine® (96 Tests). Quantitate Mouse GC in cell culture supernatants, cell lysates, serum, plasma (heparin), saliva and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Vitamin D-binding protein; DBP; VDB; Gc protein-derived macrophage activating factor; Gc-MAF; GcMAF; Gc-globulin; Group-specific component; Gc; Vitamin D-binding protein-macrophage activating factor; DBP-maf; GC

Immunogen

Expression system for standard: NS0; Immunogen sequence: L17-S476

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Vitamin DBP

Subcellular Localization

Secreted.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma, Saliva, Urine

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	551	2528	8207	568	2328	8686
Standard deviation	36.91	156.73	648.35	47.14	144.33	773.05
CV (%)	6.7%	6.2%	7.9%	8.3%	6.2%	8.9%

Protein Function

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.

Background

Vitamin D-binding protein (DBP), also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene. It is mapped to 5 E1; 5 44.32 cM. The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	551	505	496	595	536	39.57	7.3%
Sample 2	2528	2531	2745	2357	2540	137.6	5.4%
Sample 3	8207	9826	9283	9589	9226	619.14	6.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	14473
Antigen	BP/GC/Vitamin
Gene Name	Gc
UniProt ID	P21614
Tissue Specificity	Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001575	EK2063	96 wells/kit	$499		Add to Cart

Mouse Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®

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