Mouse Vinculin - VCL ELISA Kit

Mfr.No.

E2563Mo

Alternative Names

9430097D22;AA571387;AI462105;AW545629;Metavinculin;VCL;Vinculin

Reactivity

Mouse

Detection Range

20-3500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

116717

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.63ng/L

Background

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).

Gene ID	22330
UniProt ID	Q64727
Antigen	VCL

Research Area

Signal Transduction

Protocol

These standard curves of E2563Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	1.717	1.824	1.795
	1.932
1000ng/L	0.886	0.895	0.865
	0.903
500ng/L	0.585	0.548	0.519
	0.511
250ng/L	0.179	0.197	0.168
	0.215
125ng/L	0.141	0.108	0.079
	0.075
0ng/L	0.032	0.029	0
	0.027

Precision

We measured random samples of E2563Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	797.43	38.98	4.89
Intra-Assay	2	18	1893.59	45.16	2.38
Intra-Assay	3	18	726.3	41.02	5.65

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110374	E2563Mo	48T	$320		Add to Cart
BHE12110374	E2563Mo	96T	$458		Add to Cart

Mouse Vinculin - VCL ELISA Kit

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