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Home / ELISA Kit

Mouse Uridine phosphorylase 1 (UPP1) ELISA Kit

SKU : BHE13705197

Uridine Phosphorylase 2 belongs to the PNP/UDP phosphorylase family.Predominantly expressed in kidney.Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1- phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Shows substrate specificity and accept uridine, deoxyuridine, and thymidine as well as the two pyrimidine nucleoside analogs 5-fluorouridine and 5-fluoro-2(')-deoxyuridine as substrates.Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine.This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase.

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Mfr.No.	KTE70035
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Uridine phosphorylase 1 (UPP1) ELISA Kit employs a two-site sandwich ELISA to quantitate UPP1.
Alternative Names	UPP1; UDRPASE; UP; UPASE; UPP; OTTHUMP00000159566
Detection Method	Colorimetric
Kit Components	Mouse Uridine phosphorylase 1 microplate Mouse Uridine phosphorylase 1 standard Mouse Uridine phosphorylase 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Uridine phosphorylase 1 (UPP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse UPP1. No significant cross-reactivity or interference between Mouse UPP1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Uridine Phosphorylase 2 belongs to the PNP/UDP phosphorylase family.Predominantly expressed in kidney.Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1- phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Shows substrate specificity and accept uridine, deoxyuridine, and thymidine as well as the two pyrimidine nucleoside analogs 5-fluorouridine and 5-fluoro-2(')-deoxyuridine as substrates.Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine.This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase.
Notes	This Mouse Uridine phosphorylase 1 (UPP1) ELISA Kit employs a two-site sandwich ELISA to quantitate UPP1 in samples. An antibody specific for UPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	22271

Applications	ELISA
Applications Notes	This Mouse Uridine phosphorylase 1 (UPP1) ELISA Kit employs a two-site sandwich ELISA to quantitate UPP1 in samples. An antibody specific for UPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705197	KTE70035	48 T	$359		Add to Cart
BHE13705197	KTE70035	96 T	$619		Add to Cart
BHE13705197	KTE70035	96 Tx5	$2809		Add to Cart
BHE13705197	KTE70035	96 Tx50	$26209		Add to Cart

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