Mouse Tumor Necrosis Factor Ligand Superfamily Member 12

Mfr.No.

E2532Mo

Sensitivity

3.28ng/L

Detection Range

6-800ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Apo3l;DR;Dr3l;Dr3lg;TNF-related weak inducer of apoptosis;TNFSF12;Tumor necrosis factor ligand superfamily member 12;Tumor necrosis factor ligand superfamily member 12, membrane form;Tumor necrosis factor ligand superfamily member 12, secreted form);Twea;Tweak

Molecular Weight

27441

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2532Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	280.73	14.09	5.02
Intra-Assay	2	18	724.42	18.48	2.55
Intra-Assay	3	18	590.85	30.7	5.2

Background

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion (By similarity).

Protocol

These standard curves of E2532Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960ng/L	1.918	1.966	1.927
	2.014
480ng/L	0.912	0.945	0.906
	0.978
240ng/L	0.435	0.498	0.459
	0.561
120ng/L	0.192	0.237	0.198
	0.282
60ng/L	0.093	0.104	0.065
	0.115
0ng/L	0.037	0.039	0
	0.041

Gene ID	21944
UniProt ID	O54907

Research Area	Cell Biology; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110343	E2532Mo	48T	$320		Add to Cart
BHE12110343	E2532Mo	96T	$458		Add to Cart

Mouse Tumor Necrosis Factor Ligand Superfamily Member 12 - TNFSF12 ELISA Kit

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