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Home / ELISA Kit

Mouse Transmembrane protease serine 2 (TMPRSS2) ELISA Kit

SKU : BHE13705312

Transmembrane protease, serine 2 protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes.
This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

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Mfr.No.	KTE70152
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transmembrane protease serine 2 (TMPRSS2) ELISA Kit employs a two-site sandwich ELISA to quantitate TMPRSS2.
Alternative Names	TMPRSS2; FLJ41954; PP9284; PRSS10; OTTHUMP00000207917; epitheliasin
Detection Method	Colorimetric
Kit Components	Mouse Transmembrane protease serine 2 microplate Mouse Transmembrane protease serine 2 standard Mouse Transmembrane protease serine 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transmembrane protease serine 2 (TMPRSS2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMPRSS2. No significant cross-reactivity or interference between Mouse TMPRSS2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Transmembrane protease, serine 2 protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Notes	This Mouse Transmembrane protease serine 2 (TMPRSS2) ELISA Kit employs a two-site sandwich ELISA to quantitate TMPRSS2 in samples. An antibody specific for TMPRSS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMPRSS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMPRSS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPRSS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	50528

Applications	ELISA
Applications Notes	This Mouse Transmembrane protease serine 2 (TMPRSS2) ELISA Kit employs a two-site sandwich ELISA to quantitate TMPRSS2 in samples. An antibody specific for TMPRSS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMPRSS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMPRSS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPRSS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705312	KTE70152	48 T	$339		Add to Cart
BHE13705312	KTE70152	96 T	$589		Add to Cart
BHE13705312	KTE70152	96 Tx5	$2629		Add to Cart
BHE13705312	KTE70152	96 Tx50	$24569		Add to Cart

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