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Home / ELISA Kit

Mouse Transmembrane and TPR repeat-containing protein 1 (TMTC1) ELISA Kit

SKU : BHE13705306

TMTC1 is a multi-pass membrane protein. It belongs to the TMTC family and contains 10 TPR repeats. The tetratrico peptide repeat (TPR) is a structural motif present in a wide range of proteins. It mediates protein-protein interactions and the assembly of multiprotein complexes. The TPR motif consists of 3-16 tandem-repeats of 34 amino acids residues, although individual TPR motifs can be dispersed in the protein sequence. Sequence alignment of the TPR domains reveals a consensus sequence defined by a pattern of small and large amino acids. TPR motifs have been identified in various different organisms, ranging from bacteria to humans. Proteins containing TPRs are involved in a variety of biological processes, such as cell cycle regulation, transcriptional control, mitochondrial and peroxisomal protein transport, neurogenesis and protein folding.

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Mfr.No.	KTE70146
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transmembrane and TPR repeat-containing protein 1 (TMTC1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMTC1.
Alternative Names	TMTC1; ARG99; FLJ31400; FLJ41625; OLF; TMTC1A; transmembrane and tetratricopeptide repeat containing 1A
Detection Method	Colorimetric
Kit Components	Mouse Transmembrane and TPR repeat-containing protein 1 microplate Mouse Transmembrane and TPR repeat-containing protein 1 standard Mouse Transmembrane and TPR repeat-containing protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transmembrane and TPR repeat-containing protein 1 (TMTC1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMTC1. No significant cross-reactivity or interference between Mouse TMTC1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TMTC1 is a multi-pass membrane protein. It belongs to the TMTC family and contains 10 TPR repeats. The tetratrico peptide repeat (TPR) is a structural motif present in a wide range of proteins. It mediates protein-protein interactions and the assembly of multiprotein complexes. The TPR motif consists of 3-16 tandem-repeats of 34 amino acids residues, although individual TPR motifs can be dispersed in the protein sequence. Sequence alignment of the TPR domains reveals a consensus sequence defined by a pattern of small and large amino acids. TPR motifs have been identified in various different organisms, ranging from bacteria to humans. Proteins containing TPRs are involved in a variety of biological processes, such as cell cycle regulation, transcriptional control, mitochondrial and peroxisomal protein transport, neurogenesis and protein folding.
Notes	This Mouse Transmembrane and TPR repeat-containing protein 1 (TMTC1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMTC1 in samples. An antibody specific for TMTC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMTC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMTC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMTC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	387314

Applications	ELISA
Applications Notes	This Mouse Transmembrane and TPR repeat-containing protein 1 (TMTC1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMTC1 in samples. An antibody specific for TMTC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMTC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMTC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMTC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705306	KTE70146	48 T	$339		Add to Cart
BHE13705306	KTE70146	96 T	$589		Add to Cart
BHE13705306	KTE70146	96 Tx5	$2629		Add to Cart
BHE13705306	KTE70146	96 Tx50	$24569		Add to Cart

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