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Home / ELISA Kit

Mouse Transmembrane 6 superfamily member 2 (TM6SF2) ELISA Kit

SKU : BHE13705359

TM9SF2 contains a putative signal sequence, a hydrophilic N-terminal region, and 9 predicted C-terminal transmembrane domains. TM9SF2 shares 35% amino acid sequence identity with the S. cerevisiae Emp70 protein. By indirect immunofluorescence microscopy, recombinant TM9SF2 appeared to be localized to endosomes by virtue of its apparent colocalization with transferrin receptors and some mannose 6-phosphate receptors; TM9SF2 was not detected in the plasma membrane or in the Golgi apparatus. Northern blot analysis detected an approximately 3.2-kb TM9SF2 transcript in all human tissues tested, with the highest expression in pancreas, high expression in kidney, lower expression in heart, brain, skeletal muscle, and placenta, and the lowest expression in lung and liver.

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Mfr.No.	KTE70199
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transmembrane 6 superfamily member 2 (TM6SF2) ELISA Kit employs a two-site sandwich ELISA to quantitate TM6SF2.
Alternative Names	TM6SF2; KIAA1926;
Detection Method	Colorimetric
Kit Components	Mouse Transmembrane 6 superfamily member 2 microplate Mouse Transmembrane 6 superfamily member 2 standard Mouse Transmembrane 6 superfamily member 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transmembrane 6 superfamily member 2 (TM6SF2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TM6SF2. No significant cross-reactivity or interference between Mouse TM6SF2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TM9SF2 contains a putative signal sequence, a hydrophilic N-terminal region, and 9 predicted C-terminal transmembrane domains. TM9SF2 shares 35% amino acid sequence identity with the S. cerevisiae Emp70 protein. By indirect immunofluorescence microscopy, recombinant TM9SF2 appeared to be localized to endosomes by virtue of its apparent colocalization with transferrin receptors and some mannose 6-phosphate receptors; TM9SF2 was not detected in the plasma membrane or in the Golgi apparatus. Northern blot analysis detected an approximately 3.2-kb TM9SF2 transcript in all human tissues tested, with the highest expression in pancreas, high expression in kidney, lower expression in heart, brain, skeletal muscle, and placenta, and the lowest expression in lung and liver.
Notes	This Mouse Transmembrane 6 superfamily member 2 (TM6SF2) ELISA Kit employs a two-site sandwich ELISA to quantitate TM6SF2 in samples. An antibody specific for TM6SF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM6SF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM6SF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM6SF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	107770

Applications	ELISA
Applications Notes	This Mouse Transmembrane 6 superfamily member 2 (TM6SF2) ELISA Kit employs a two-site sandwich ELISA to quantitate TM6SF2 in samples. An antibody specific for TM6SF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM6SF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM6SF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM6SF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705359	KTE70199	48 T	$339		Add to Cart
BHE13705359	KTE70199	96 T	$589		Add to Cart
BHE13705359	KTE70199	96 Tx5	$2629		Add to Cart
BHE13705359	KTE70199	96 Tx50	$24569		Add to Cart

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