Mouse Transforming growth factor-beta-induced protein ig-h3(TGFBI) ELISA kit

Mouse Transforming growth factor-beta-induced protein ig-h3(TGFBI) ELISA kit

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Mfr.No.

CSB-EL023450MO

Sensitivity

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Detection Range

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Reactivity

Mouse

Assay Duration

1-5h

Alternative Names

transforming growth factor, beta-induced, 68kDa, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1, RGD-containing collagen-associated protein, kerato-epithelin

Recovery

The recovery of human WNT3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-98
EDTA plasma (n=4)	96	90-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human WNT3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-98
1:2	Average %	95
	Range %	90-100
1:4	Average %	102
	Range %	91-110
1:8	Average %	92
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.030	1.952	1.991	1.823
5	1.549	1.476	1.513	1.345
2.5	1.092	1.014	1.053	0.885
1.25	0.712	0.709	0.711	0.543
0.625	0.487	0.449	0.468	0.300
0.312	0.372	0.359	0.366	0.198
0.156	0.273	0.254	0.264	0.096
0	0.170	0.166	0.168	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P82198

Applications	ELISA
Research Area	TGFBI

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506820	CSB-EL023450MO	96T	$595	Add to Cart
BHE10506820	CSB-EL023450MO	5x96T	$2856	Add to Cart
BHE10506820	CSB-EL023450MO	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.