Mouse Transforming Growth Factor alpha

Mfr.No.

E0238Mo

Description

This sandwich kit is for the accurate quantitative detection of Mouse Transforming Growth Factor Alpha (also known as TGF-α) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

EGF-like TGF;ETGF;Protransforming growth factor alpha Transforming growth factor alpha;TGF A;TGF alpha;TGF type 1;TGFA;TGF-alpha

Reactivity

Mouse

Detection Range

1-100ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

17018

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.64ng/L

Background

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Gene ID	21802
UniProt ID	P48030
Antigen	TGFA

Research Area

Signal Transduction

Protocol

These standard curves of E0238Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/L	2.187	2.159	2.124
	2.131
32ng/L	1.139	1.235	1.2
	1.331
16ng/L	0.774	0.737	0.701
	0.699
8ng/L	0.385	0.418	0.383
	0.451
4ng/L	0.198	0.2	0.165
	0.201
0ng/L	0.045	0.035	0
	0.025

Precision

We measured random samples of E0238Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.3	0.45	5.5
Intra-Assay	2	18	20.2	0.99	4.9
Intra-Assay	3	18	44.7	1.54	3.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108151	E0238Mo	48T	$320		Add to Cart
BHE12108151	E0238Mo	96T	$458		Add to Cart

Mouse Transforming Growth Factor alpha - TGF-A ELISA Kit

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