Mouse TNFRSF9/4-1BB ELISA Kit PicoKine®

Mfr.No.

EK1143

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.036	0.154	0.224	0.361	0.582	1.149	1.815	2.401

Description

Mouse TNFRSF9/4-1BB ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 9;4-1BB ligand receptor;T-cell antigen 4-1BB;CD137;Tnfrsf9;Cd137, Ila, Ly63;

Molecular Weight

27598 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: V24-L187

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF9

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	88	292	930	88	292	917
Standard deviation	6%6	15.82	70.68	6.95	17.52	87.11
CV (%)	6.7%	5.4%	7.6%	7.9%	6%	9.5%

Protein Function

Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Background

CD137 is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB and induced by lymphocyte activation (ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	95	83	93	86	89	4.91	5.5%
Sample 2	293	265	248	285	272	17.55	6.4%
Sample 3	930	876	954	952	928	31.46	3.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	21942
Antigen	TNFRSF9/4-1BB
Gene Name	TNFRSF9
UniProt ID	P20334
Protein Name	Tumor necrosis factor receptor superfamily member 9
Tissue Specificity	Expressed on the surface of activated T-cells.

Applications	ELISA
Research Area	Apoptosis,Associated Proteins,Cancer,Cell Biology,Cell Death,Cell Type Markers,Cytokines,Hematopoietic Progenitors,Immunology,Innate Immunity,Lymphoid,Receptors,Stem Cells,T Lymphocytic Lineage,TNF Superfamily,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000789	EK1143	96 wells/kit	$499		Add to Cart

Mouse TNFRSF9/4-1BB ELISA Kit PicoKine®

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