Mouse TNFRSF9/4-1BB ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ1143

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.039	0.066	0.122	0.206	0.372	0.748	1.375	2.121

Description

Mouse TNFRSF9/4-1BB ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Tnfrsf9 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA).

Alternative Names

Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; T-cell antigen 4-1BB; CD137; Tnfrsf9; Cd137; Ila; Ly63

Immunogen

Expression system for standard: NS0; Immunogen sequence: V24-L187

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF9/4-1BB

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	319	746	1253	286	833	1353
Standard deviation	12.76	28.3	66.5	16.6	37.5	94.7
CV (%)	4%	3.8%	5.3%	5.8%	4.5%	7%

Protein Function

Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Background

CD137 is a member of the tumor necrosis factor (TNF) receptor family. Its alteative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB and induced by lymphocyte activation (ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	21942
Gene Name	Tnfrsf9
UniProt ID	P20334
Tissue Specificity	Expressed on the surface of activated T-cells.

Applications	ELISA
Research Area	Apoptosis,Associated Proteins,Cancer,Cell Biology,Cell Death,Cell Type Markers,Cytokines,Hematopoietic Progenitors,Immunology,Innate Immunity,Lymphoid,Receptors,Stem Cells,T Lymphocytic Lineage,TNF Superfamily,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000041	EZ1143	5 plates/kit	$500		Add to Cart

Mouse TNFRSF9/4-1BB ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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