Mouse TNFRSF7/CD27 ELISA Kit PicoKine®

Mfr.No.

EK0699

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.000	0.181	0.286	0.531	0.967	1.464	1.905	2.199

Description

Mouse TNFRSF7/CD27 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd27 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CD27 antigen;CD27L receptor;T-cell activation antigen CD27;Tumor necrosis factor receptor superfamily member 7;CD27;Cd27;Tnfrsf7;

Molecular Weight

28164 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: T21-R182

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF7

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	83	588	1891	74	556	2053
Standard deviation	3.81	45.86	115.35	3.55	51.15	149.86
CV (%)	4.6%	7.8%	6.1%	4.8%	9.2%	7.3%

Protein Function

Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Background

CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	83	88	88	94	88	3.89	4.4%
Sample 2	588	678	590	656	628	39.77	6.3%
Sample 3	1891	1745	2088	2100	1956	147.39	7.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	21940
Antigen	TNFRSF7/CD27
Gene Name	CD27
UniProt ID	P41272
Protein Name	CD27 antigen
Tissue Specificity	In thymus and spleen, but not in non-lymphoid tissues.

Applications	ELISA
Research Area	Adaptive Immunity,Cancer,Cytokines,Hematopoietic Progenitors,Immunology,Innate Immunity,Lymphoid,Stem Cells,T Cells,T Lymphocytic Lineage,TNF Superfamily,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000573	EK0699	96 wells/kit	$499		Add to Cart

Mouse TNFRSF7/CD27 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse TNFRSF7/CD27 ELISA Kit PicoKine®

Rate it now..!!