Mouse TNFRSF25/DR3 ELISA Kit PicoKine®

Mfr.No.

EK1515

Sensitivity

<15 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.041	0.092	0.122	0.195	0.401	0.847	1.741	2.402

Description

Mouse TNFRSF25/DR3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf25 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Protein Tnfrsf25 ;Tumor necrosis factor receptor superfamily, member 25, isoform CRA_b ;Tnfrsf25 ;mCG_4090 ;

Molecular Weight

44264 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q31-F199

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF25

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	206	1289	4332	201	1293	3972
Standard deviation	8.44	68.31	316.23	11.65	68.52	317.76
CV (%)	4.1%	5.3%	7.3%	5.8%	5.3%	8%

Background

Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alteatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alteative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	206	209	222	189	206	11.75	5.7%
Sample 2	1289	1163	1209	1152	1203	53.92	4.4%
Sample 3	4332	4412	4054	4654	4363	214.19	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	85030
Gene Name	Tnfrsf25
UniProt ID	B1AWN9
Protein Name	Protein Tnfrsf25

Applications	ELISA
Research Area	Apoptosis,Cell Biology,NFKB Pathway,Nuclear Signaling,Receptors,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001089	EK1515	96 wells/kit	$750		Add to Cart

Mouse TNFRSF25/DR3 ELISA Kit PicoKine®

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