Mouse TNFRSF13C/BAFFR ELISA Kit PicoKine®

Mfr.No.

EK1122

Sensitivity

<10 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.022	0.087	0.133	0.228	0.411	0.747	1.44	2.227

Description

Mouse TNFRSF13C/BAFFR ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf13c in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 13C;B-cell maturation defect;B-cell-activating factor receptor;BAFF receptor;BAFF-R;BLyS receptor 3;CD268;Tnfrsf13c;Baffr, Bcmd, Br3;

Molecular Weight

18798 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S10-A71

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF13C

Subcellular Localization

Membrane ; Single-pass type III membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	110	605	2620	117	626	2583
Standard deviation	4.4	47.19	117.9	7.8%1	60.72	154.98
CV (%)	4%	7.8%	4.5%	4.8%	9.7%	6%

Protein Function

B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. .

Background

Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	110	112	109	105	109	2.54	2.3%
Sample 2	605	673	577	616	617	34.92	5.6%
Sample 3	2620	2839	2527	2525	2627	127.86	4.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	72049
Gene Name	TNFRSF13C
UniProt ID	Q9D8D0
Protein Name	Tumor necrosis factor receptor superfamily member 13C
Tissue Specificity	Highly expressed in spleen and testis; detected at lower levels in lung and thymus.

Applications	ELISA
Research Area	Growth Factors/Hormones,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000776	EK1122	96 wells/kit	$499		Add to Cart

Mouse TNFRSF13C/BAFFR ELISA Kit PicoKine®

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