Mouse TNFRSF13B/TACI ELISA Kit PicoKine®

Mfr.No.

EK1144

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	46.9	94	188	375	750	1500	3000
O.D.	0.032	0.097	0.154	0.235	0.409	0.722	1.329	2.243

Description

Mouse TNFRSF13B/TACI ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf13b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 13B;Transmembrane activator and CAML interactor;CD267;Tnfrsf13b;Taci;

Molecular Weight

26947 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A2-C128

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TNFRSF13B

Subcellular Localization

Membrane ; Single-pass type III membrane protein .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	88	327	1327	96	311	1224
Standard deviation	4.92	22.89	74.31	7.1	22.7	88.12
CV (%)	7%%	7%	5.6%	7.4%	7.3%	7.2%

Protein Function

Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T- cell function and the regulation of humoral immunity (By similarity). .

Background

Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	88	91	89	95	90	2.68	2.9%
Sample 2	327	381	369	367	361	20.34	5.6%
Sample 3	1327	1381	1420	1248	1344	64.51	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	57916
Antigen	TNFRSF13B/TACI
Gene Name	TNFRSF13B
UniProt ID	Q9ET35
Protein Name	Tumor necrosis factor receptor superfamily member 13B

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000790	EK1144	96 wells/kit	$499		Add to Cart

Mouse TNFRSF13B/TACI ELISA Kit PicoKine®

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