Mouse TNF Beta ELISA Kit PicoKine®

Mfr.No.

EK0585

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.035	0.081	0.140	0.283	0.525	1.036	1.468	2.011

Description

Mouse TNF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lta in cell culture supeatants, serum and plasma (heparin, EDTA or citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

LT, LT-(a), LT-alpha, LT(a), LTalpha, Ltx, TNF-beta, TNFSF1, Tnfb, Tnfsf1b, Tnlg1e, hlb382

Molecular Weight

22 kDa

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L34-L202

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Lta

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	91	379	1647	85	460	1810
Standard deviation	1.83/td>	22.10	98.33	2.48	29.30	110.05
CV (%)	2.0%	5.8%%	6.0%	2.9%	6.4%	6.1%

Background

The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alteatively spliced transcript variants have been observed for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	91	106	93	87	94	2.13	2.3%
Sample 2	379	322	301	352	339	18.35	5.4%
Sample 3	1647	1895	1224	1470	1559	46.15	3.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16992
Gene Name	Lta
UniProt ID	P09225
Protein Name	Lymphotoxin-alpha

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001904	EK0585	96 wells/kit	$499		Add to Cart

Mouse TNF Beta ELISA Kit PicoKine®

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