Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine®

Mfr.No.

EK1130

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.016	0.078	0.128	0.200	0.412	0.853	1.434	2.097

Description

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tlr2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Toll-like receptor 2;CD282;Tlr2;

Molecular Weight

89449 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q25-L590

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TLR2

Subcellular Localization

Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	304	1448	4661	301	1423	4925
Standard deviation	21.58	65.76	195.76	26.48	91.07	280.72
CV (%)	7.1%	4.5%	4.2%	8.8%	6.4%	5.7%

Protein Function

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins (By similarity). .

Background

Toll-like receptor 2 also known as TLR-2 is a protein that in humans is encoded by the TLR2 gene. TLR2 has also been designated as CD282 (cluster of differentiation 282). TLR-2 plays a role in the immune system. TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	304	323	310	307	311	7.24	2.3%
Sample 2	1448	1379	1487	1331	1411	60.35	4.2%
Sample 3	4661	5150	4522	4797	4782	233.39	4.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	24088
Gene Name	TLR2
UniProt ID	Q9QUN7
Protein Name	Toll-like receptor 2
Tissue Specificity	Detected in a macrophage cell line, smooth muscle, lung, spleen, thymus, brain and adipose tissue.
Sequence	Belongs to the Toll-like receptor family.

Applications	ELISA
Research Area	Adapters,Atherosclerosis,Cardiovascular,Defensin,Human Protein,Immunoglobulins,Immunology,Innate Immunity,Macrophage/Inflammation,Microbiology,Protein,Receptors,Signal Transduction,TLR Signaling,Transmembrane,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000782	EK1130	96 wells/kit	$499		Add to Cart

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine®

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