Mouse Tissue Factor/F3 ELISA Kit PicoKine®

Mfr.No.

EK1367

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.041	0.092	0.171	0.252	0.396	0.707	1.105	1.849

Description

Mouse Tissue Factor/F3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse F3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tissue factor;TF;Coagulation factor III;CD142;F3;Cf-3, Cf3;

Molecular Weight

32935 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A29-E251

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Tissue factor

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum, Plasma, Cell lysates

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	31	167	444	32	169	432
Standard deviation	1.3	9.18	32.41	1.5	10.47	35.85
CV (%)	4.2%	5.5%	7.3%	4.7%	6.2%	8.3%

Protein Function

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The (TF:VIIa) complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Background

Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	31	36	32	30	32	2.27	7%
Sample 2	167	171	178	166	170	4.71	2.7%
Sample 3	444	389	387	433	413	25.55	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	14066
Antigen	Factor/F3
Gene Name	F3
UniProt ID	P20352
Protein Name	Tissue factor

Applications	ELISA
Research Area	Cardiovascular

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000958	EK1367	96 wells/kit	$499		Add to Cart

Mouse Tissue Factor/F3 ELISA Kit PicoKine®

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