Mouse TIMP-4 ELISA Kit PicoKine®

Mfr.No.

EK1335

Sensitivity

<10 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.064	0.169	0.232	0.339	0.567	0.965	1.465	1.947

Description

Mouse TIMP-4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Timp4 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Metalloproteinase inhibitor 4;Tissue inhibitor of metalloproteinases 4;TIMP-4;Timp4;

Molecular Weight

25774 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: C30-P224

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TIMP-4

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	131	690	2000	137	710	2055
Standard deviation	6.94	44.85	156	8.63	51.83	199.33
CV (%)	5.3%	6.5%	7.8%	7.3%%	7.3%	9.7%

Protein Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Background

Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. Whats more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	131	113	132	117	123	8.37	6.8%
Sample 2	690	674	647	709	680	22.72	3.3%
Sample 3	2000	2212	1975	2086	2068	92.64	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	110595
Gene Name	TIMP4
UniProt ID	Q9JHB3
Protein Name	Metalloproteinase inhibitor 4
Tissue Specificity	Expressed in brain, heart, ovary and skeletal muscle.
Sequence	Belongs to the protease inhibitor I35 (TIMP) family.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Cell Biology,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Protease Inhibitors,Proteolysis/Ubiquitin

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000931	EK1335	96 wells/kit	$499		Add to Cart

Mouse TIMP-4 ELISA Kit PicoKine®

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