Mouse TGF-Beta 3 ELISA Kit PicoKine®

Mfr.No.

EK1104

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.025	0.065	0.105	0.175	0.311	0.657	1.054	1.877

Description

Mouse TGF-Beta 3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tgfb3 in cell culture supeatants, serum, plasma (EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Transforming growth factor beta-3;TGF-beta-3;Latency-associated peptide;LAP;Tgfb3;

Molecular Weight

46885 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A301-S412

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TGF-beta 3

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Assay Procedure Summary

Activate the sample

Cell culture supeatants, urine: add activating reagent pro rata, i.e. add 20?l of Solution A into 100?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6.

Serum, plasma(EDTA): add activating reagent pro rata, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6.

It is unnecessary to activate the recombinant TGF-beta 3.

Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Anticoagulant

EDTA

Activating Reagent

TGF beta 3 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant TGF beta 3.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H₂O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H₂O, add H₂O to adjust volume to 100ml.

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	58	278	1013	63	286	988
Standard deviation	2.61	15.01	45.58	3.59	15.70	57.3
CV (%)	4.5%	5.4%	4.5%	5.7%	5.7%	5.8%

Protein Function

Involved in embryogenesis and cell differentiation.

Background

Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	58	64	58	53	58	3.89	6.7%
Sample 2	278	301	266	300	286	14.87	5.1%
Sample 3	1013	982	950	928	968	32.18	3.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	21809
Gene Name	TGFB3
UniProt ID	P17125
Protein Name	Transforming growth factor beta-3
Tissue Specificity	Expressed in both testis and ovary.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Growth Factors,Growth Factors/Hormones,Invasion/Microenvironment,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000763	EK1104	96 wells/kit	$499		Add to Cart

Mouse TGF-Beta 3 ELISA Kit PicoKine®

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