Mouse TGF-Beta 2 ELISA Kit PicoKine®

Mfr.No.

EK0983

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.008	0.168	0.197	0.230	0.350	0.565	0.938	1.815

Description

Mouse TGF-Beta 2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tgfb2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Transforming growth factor beta-2;TGF-beta-2;Latency-associated peptide;LAP;Tgfb2;

Molecular Weight

47589 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A303-S414

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse TGF-beta 2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Assay Procedure Summary

Activate the sample

Cell culture supeatants: add activating reagent pro rata, i.e. add 20?l of Solution A into 100?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6.

Serum, plasma(heparin, EDTA or citrate): add activating reagent pro rata, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6.

It is unnecessary to activate the recombinant TGF-beta 2.

Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Anticoagulant

heparin, EDTA or citrate

Activating Reagent

TGF beta 2 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant TGF beta 2.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H₂O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H₂O, add H₂O to adjust volume to 100ml.

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	98	235	827	102	215	805
Standard deviation	6.46	11.98	54.58	7.54	11.82	64.4
CV (%)	6.6%	8%	6.6%	7.4%	5.5%	8%

Protein Function

TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.

Background

Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	98	90	99	91	94	4.03	4.2%
Sample 2	235	221	227	238	230	6.68	2.9%
Sample 3	827	847	843	932	862	40.95	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	21808
Gene Name	TGFB2
UniProt ID	P27090
Protein Name	Transforming growth factor beta-2

Applications	ELISA
Research Area	Metabolism Processes,Organogenesis,Pathways and Processes,Response To Hypoxia,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000746	EK0983	96 wells/kit	$499		Add to Cart

Mouse TGF-Beta 2 ELISA Kit PicoKine®

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