Mouse stromal cell derived factor 1alpha(SDF-1alpha/SDF1A)ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027494MO

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

stromal cell derived factor 1alpha(SDF-1alpha/SDF1A), stromal cell derived factor 1 alpha,SDF-1 alpha/SDF1A

Recovery

The recovery of human C20orf186 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	96-105
EDTA plasma (n=4)	93	88-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C20orf186 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	98
	Range %	92-106
1:4	Average %	84
	Range %	81-89
1:8	Average %	91
	Range %	83-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sample Type

Serum, Cell culture supernatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P40224
Tissue Specificity	Highest expression levels detected in kidney, liver, spleen and muscle. Isoform Alpha is expressed ubiquitously but at varying levels, while isoform Beta displays tissue-specific expression, with expression detected in kidney, liver, heart, spleen and mus

Applications	ELISA
Research Area	SDF1A, CXCL12

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Erythropoietin improves the efficiency of endothelial progenitor cell therapy after myocardial infarction in mice: Effects on transplanted cell survival and autologous &hellip Cheng Y et al,Journal of Surgical Research,2012 Jul;176(1):e47-55. Effect of Acute Lung Injury on VLA-4 and CXCR4 Expression in Resident and Circulating Hematopoietic Stem/Progenitor Cells Trotta T et al,Respiration,2013 85(3):252-64.

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