Mouse soluble CD6 ELISA Kit PicoKine®

Mfr.No.

EK1287

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.014	0.059	0.1	0.185	0.349	0.666	1.217	2.030

Description

Mouse soluble CD6 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd6 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

T-cell differentiation antigen CD6;CD6;Cd6;

Molecular Weight

72255 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L18-G396

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD6

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	133	525	1268	129	485	1288
Standard deviation	5.58	38.9%7	92.56	6.45	37.83	114.63
CV (%)	4.2%	6.7%	7.3%	5%	7.8%	8.9%

Protein Function

Involved in cell adhesion. Binds to CD166.

Background

CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. Whats more, this gene may be associated with susceptibility to multiple sclerosis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	133	133	120	119	126	6.75	5.3%
Sample 2	525	507	540	469	510	26.52	5.2%
Sample 3	1268	1267	1220	1339	1273	42.5	3.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12511
Gene Name	CD6
UniProt ID	Q61003
Protein Name	T-cell differentiation antigen CD6
Tissue Specificity	Expressed predominantly in thymus, lymph node and spleen.

Applications	ELISA
Research Area	Adaptive Immunity,Hematopoietic Progenitors,Immunology,Lymphoid,Stem Cells,T Cells,T Lymphocytic Lineage

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000876	EK1287	96 wells/kit	$499		Add to Cart

Mouse soluble CD6 ELISA Kit PicoKine®

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