Mouse SEZ6L ELISA Kit PicoKine®

Mfr.No.

EK1863

Sensitivity

<50 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.052	0.164	0.223	0.316	0.552	0.938	1.413	2.174

Description

Mouse SEZ6L ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Sez6l in cell culture supeatants, serum and plasma (heparin). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Seizure 6-like protein; Acupuncture-induced protein 1-L; Brain-specific receptor-like protein B; BSRP-B; Sez6l; Aig1l; Kiaa0927

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-A958

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse SEZ6L

Subcellular Localization

Cell membrane. Single-pass type I membrane protein. Endoplasmic reticulum membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	119	516	2469	129	562	2481
Standard deviation	5.71	27.35	138.26	6.58	32.03	153.82
CV (%)	4.8%	5.3%	5.6%	5.1%	5.7%	6.2%

Protein Function

Candidate tumor suppressor gene. May contribute to specialized endoplasmic reticulum functions in neurons.

Background

Seizure 6-like protein is a protein that in humans is encoded by the SEZ6L gene. It is mapped to 5; 5 F.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	119	109	109	125	115	6.83	5.9%
Sample 2	516	625	523	552	554	43.15	7.7%
Sample 3	2469	2400	2352	2325	2386	54.68	2.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	56747
Gene Name	Sez6l
UniProt ID	Q6P1D5
Tissue Specificity	Expressed exclusively in the brain, predomimantly in neurons. Wide expression in the gray matter of the brain with high levels in the olfactory bulb, anterior olfactory nuclei, hippocampal formation and cerebellar cortex. Detected diffusely and weakly in the white matter, such as the corpus callosum and cerebellar medulla. In the cerebellar cortex, intensely expressed in Purkinje cells and granule cells. Detected also in inteeurons in the molecular layer.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001622	EK1863	96 wells/kit	$750		Add to Cart

Mouse SEZ6L ELISA Kit PicoKine®

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