Mouse SerpinE2 ELISA Kit PicoKine®

Mfr.No.

EK1885

Sensitivity

<0.1 ng/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.089	0.125	0.181	0.287	0.449	0.886	1.85	2.043

Description

Mouse SerpinE2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpine2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate) ,urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Glia-derived nexin; GDN; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serine protease-inhibitor 4; Serpin E2; Serpine2; Pi7; Pn1; Spi4

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-P397

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse SerpinE2

Subcellular Localization

extracellular space

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	599	2848	10128	604	2990	10197
Standard deviation	33.54	165.18	455.76	36.84	191.36	723.99
CV (%)	5.6%	5.8%	4.5%	6.1%	6.4%	7.1%

Protein Function

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.

Background

Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 1 C4; 1 40.97 cM. SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	599	657	572	652	620	35.83	5.7%
Sample 2	2848	2654	2960	2710	2793	119.50	4.2%
Sample 3	10128	10127	9926	9025	9801	455.79	4.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20720
Gene Name	Serpine2
UniProt ID	Q07235
Tissue Specificity	Most abundant in seminal vesicles.

Applications	ELISA
Research Area	Cardiovascular,Cell Adhesion Proteins,Cell Biology,Neurogenesis,Neurology Process,Neuroscience,Proteolysis/Ubiquitin,Proteolytic Enzymes

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001430	EK1885	96 wells/kit	$750		Add to Cart

Mouse SerpinE2 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse SerpinE2 ELISA Kit PicoKine®

Rate it now..!!