Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®

Mfr.No.

EK1713

Sensitivity

<10 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (ng/ml)	0	1.56	3.13	6.25	12.5	25	50	100
O.D.	0.066	0.122	0.175	0.276	0.520	0.952	1.408	2.380

Description

Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpinf2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Alpha-2-antiplasmin; Alpha-2-AP; Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2; Serpinf2; Pli

Immunogen

Expression system for standard: NS0; Immunogen sequence: N40-K491

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse GPC3

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2710	13864	42376	2900	14200	45464
Standard deviation	113.82	804.1	2712.06	130.5	965.6	3273.4
CV (%)	4.2%	5.8%	6.4%	4.5%	6.8%	7.2%

Protein Function

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin (By similarity). .

Background

Alpha-2-antiplasmin is a serine protease inhibitor (serpin) responsible for inactivating plasmin. Plasmin is an important enzyme that participates in fibrinolysis and degradation of various other proteins. This protein is encoded by the SERPINF2 gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2710	2230	2706	2277	2480	227.86	9.1%
Sample . 2	13864	12175	13380	12105	12881	760.90	5.9%%
Sample 3	42376	42947	47567	46317	44801	2194.70	4.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	18816
Gene Name	Serpinf2
UniProt ID	Q61247
Tissue Specificity	Expressed by the liver and secreted in plasma.

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Cell Biology,Neurogenesis,Neurology Process,Neuroscience,Protease Inhibitors,Proteolysis/Ubiquitin,Sensory System,Visual System

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001369	EK1713	96 wells/kit	$499		Add to Cart

Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®

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