Mouse Serpin D1 ELISA Kit PicoKine®

Mfr.No.

EK1969

Sensitivity

<0.1 ng/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.033	0.328	0.448	0.857	1.077	1.482	1.954	2.310

Description

Mouse Serpin D1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpind1 in cell culture supeatants, serum and plasma (EDTA, citrate) and urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Heparin cofactor 2; Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; Serpin D1; Serpind1; Hcf2; Hcii

Immunogen

Expression system for standard: NS0; Immunogen sequence: E24-S478

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Serpin D1

Subcellular Localization

Extracellular space.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1219	5460	26552	1229	6087	28436
Standard deviation	54.86	354.90	1778.98	83.57	359.13	1734.60
CV (%)	4.5%	6.5%	6.7%	6.8%	5.9%	6.1%

Protein Function

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT). Also inhibits chymotrypsin, but in a glycosaminoglycan-

Background

Heparin cofactor II (HCII), a protein encoded by the SERPIND1 gene, is a coagulation factor that inhibits IIa, and is a cofactor for heparin and dermatan sulfate. It is mapped to 16 A3; 16 10.74 cM. This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1219	1130	1188	1149	1171	34.48	2.9%
Sample 2	5460	5176	5677	5786	5524	233.05	4.2%
Sample 3	26552	26436	26634	25685	26326	377.13	1.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	15160
Gene Name	Serpind1
UniProt ID	P49182
Tissue Specificity	Expressed predominantly in liver.

Applications	ELISA
Research Area	Cardiovascular

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001474	EK1969	96 wells/kit	$499		Add to Cart

Mouse Serpin D1 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse Serpin D1 ELISA Kit PicoKine®

Rate it now..!!