Mouse Serpin A1 ELISA Kit PicoKine®

Mfr.No.

EK1711

Sensitivity

<10 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (ng/ml)	0	1.56	3.13	6.25	12.5	25	50	100
O.D.	0.042	0.207	0.368	0.659	1.054	1.369	1.645	2.069

Description

Mouse Serpin A1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpina1c in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Serpina1c; Dom3; Dom6; Spi1-3; Spi1-6; Alpha-1-antitrypsin 1-3; Alpha-1 protease inhibitor 3; Alpha-1 protease inhibitor 6; Alpha-1-antitrypsin 1-6; Serine protease inhibitor 1-3; Serine protease inhibitor 1-6; Serine protease inhibitor A1c; Serpin A1c

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-K413

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse AAT

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2876	13987	37231	2911	14122	4269
Standard deviation	158.18	713.3	1600.9	177.57	932.05	320.18
CV (%)	5.5%	5.1%	4.3%	6.1%	6.6%	7.5%

Protein Function

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.

Background

SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2876	2791	2438	2616	2680	168.37	6.2%
Sample . 2	13987	13721	13461	14393	13890	344.60	2.4%%
Sample 3	37231	37794	38247	41965	38809	1857.18	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20702
Gene Name	Serpina1c
UniProt ID	Q00896

Applications	ELISA
Research Area	Surface Molecules,Transcription,Transcription Factors,Transcription Factors/Regulators

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001370	EK1711	96 wells/kit	$499		Add to Cart

Mouse Serpin A1 ELISA Kit PicoKine®

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