Mouse Semaphorin-7A - SEMA7A ELISA Kit

Mfr.No.

E2454Mo

Alternative Names

2900057C09Rik;CD antigen CD108;CDw108;H-Sema K1;H-Sema-L;Se;sema K1;sema L;SEMA7A;Semal;Semaphorin-7A;Semaphorin-K1;Semaphorin-L;

Reactivity

Mouse

Detection Range

20-4500ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

74994

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.85ng/L

Background

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.

Gene ID	20361
UniProt ID	Q9QUR8
Antigen	SEMA7A

Research Area

Others

Protocol

These standard curves of E2454Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.884	1.952	1.909
	2.021
1200ng/L	1.053	1.008	0.965
	0.963
600ng/L	0.464	0.447	0.404
	0.429
300ng/L	0.168	0.227	0.184
	0.286
150ng/L	0.149	0.124	0.081
	0.098
0ng/L	0.027	0.043	0
	0.059

Precision

We measured random samples of E2454Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1240.91	62.12	5.01
Intra-Assay	2	18	2015.95	55.29	2.74
Intra-Assay	3	18	472.38	17.13	3.63

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110265	E2454Mo	48T	$320		Add to Cart
BHE12110265	E2454Mo	96T	$458		Add to Cart

Mouse Semaphorin-7A - SEMA7A ELISA Kit

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