Mouse sE-Selectin PicoKine® Quick ELISA Kit

Mfr.No.

FEK0502

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.013	0.041	0.080	0.201	0.368	0.751	1.474	2.156

Description

Mouse sE-Selectin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Sele in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

E-selectin;CD62 antigen-like family member E;Endothelial leukocyte adhesion molecule 1;ELAM-1;Leukocyte-endothelial cell adhesion molecule 2;LECAM2;CD62E;Sele;Elam-1;

Molecular Weight

66750 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: W22-P557

Kit Components

Description Quantity
96-well plate 1
lyophilized Standard
Biotinylated detection antibody (50x) 100 ul
Avidin-Biotin-Peroxidase Complex (30x) 400 µl
Sample diluent 30 ml
Antibody diluent 12 ml
Avidin-Biotin-Peroxidase Diluent 12 ml
Wash Buffer (25x) 20 ml
Color Developing Reagent (TMB) 10 ml
Stop Solution 10 ml
Plate Sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse sE-Selectin

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	272	1580	4329	277	1451	4721
Standard deviation	11.96	105.86	186.14	16.62	108.82	250.21
CV (%)	4.4%	6.7%	4.3%	6%	7.5%	5.3%

Protein Function

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.

Background

E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	272	280	290	284	281	6.53	2.3%
Sample 2	1580	1395	1401	1536	1478	81.52	5.5%
Sample 3	4329	4374	4283	4168	4288	76.65	1.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20339
Antigen	sE-Selectin
Gene Name	Sele
UniProt ID	Q00690
Protein Name	E-selectin
Sequence	Belongs to the selectin/LECAM family.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000146	FEK0502	96 wells/kit	$499		Add to Cart

Mouse sE-Selectin PicoKine® Quick ELISA Kit

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