Mouse SDF-1/CXCL12 ELISA Kit PicoKine®

Mfr.No.

EK0500

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
O.D.	0.039	0.072	0.119	0.198	0.431	0.675	1.352	2.023

Description

Mouse SDF-1/CXCL12 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse CXCL12 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Stromal cell-derived factor 1 ;Cxcl12 ;

Molecular Weight

13634 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: K22-M93

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Cxcl12

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	118	692	1469	115	707	1562
Standard deviation	5.31	49.13	60.23	5.64	36.76	90.6
CV (%)	4.5%	7.1%	4.1%	4.9%	5.2%	5.8%

Background

SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). This gene is located on chromosome 10q11.1. SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alteate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes. CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes. CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide. The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	118	122	115	109	116	4.18	3.6%
Sample 2	692	705	712	699	702	42.82	6.1%
Sample 3	1469	1455	1545	1547	1504	82.72	5.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20315
Gene Name	CXCL12
UniProt ID	H7BX38
Protein Name	Stromal cell-derived factor 1

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000942	EK0500	96 wells/kit	$499		Add to Cart

Mouse SDF-1/CXCL12 ELISA Kit PicoKine®

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