Mouse SCGB3A1 ELISA Kit PicoKine®

Mfr.No.

EK1694

Sensitivity

<5 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.099	0.164	0.220	0.342	0.556	0.994	1.631	2.053

Description

Mouse SCGB3A1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Scgb3a1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Secretoglobin family 3A member 1; Cytokine HIN-1; High in normal 1; Pneumo secretory protein 2; PnSP-2; Uteroglobin-related protein 2; Scgb3a1; Hin1; Pnsp2; Ugrp2

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F22-G104

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse SCGB3A1

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	37	194	396	36	197	415
Standard deviation	2.73	12.8	21.78	3.06	13.59	24.9
CV (%)	7.4%	6.6%	5.5%	8.5%	6.9%	6%

Protein Function

Secreted cytokine-like protein. Inhibits cell growth in vitro.

Background

Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. This gene encodes a small secreted protein that may function in the inhibition of cell growth. Expression of the related gene in humans is downregulated via DNA methylation in breast cancers and other tumor types. Alteatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	37	39	42	37	38	2.04	5.3%
Sample 2	194	168	184	189	183	9.75	5.3%
Sample 3	396	433	480	419	432	30.7	7.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	68662
Gene Name	Scgb3a1
UniProt ID	Q920D7
Tissue Specificity	Highly expressed in lung, where it localizes to epithelial cells lining the trachea and bronchi (PubMed:12406855, PubMed:12175512). Expression in lung is mainly restricted to bronchi, submucosal glands of the trachea, and tracheal epithelium, with little expression in terminal bronchioles (PubMed:12406855). Expressed in uterus where it localizes to epithelial cells of the uterine glands (PubMed:12175512). Also detected in heart, stomach and small intestine (PubMed:12175512).

Applications	ELISA
Research Area	Cancer,Cell Biology,Cell Cycle,Cell Cycle Inhibitors,Cytokines,Growth Factors/Hormones,Immunology,Innate Immunity,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001358	EK1694	96 wells/kit	$750		Add to Cart

Mouse SCGB3A1 ELISA Kit PicoKine®

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