Mouse SAP/PTX2/APCS PicoKine® Quick ELISA Kit

Mfr.No.

FEK1208

Sensitivity

<50 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.002	0.065	0.127	0.246	0.436	0.787	1.246	1.831

Description

Mouse SAP/PTX2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Apcs in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Serum amyloid P-component;SAP;Apcs;Ptx2, Sap;

Molecular Weight

26247 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-D224

Kit Components

Description Quantity
96-well plate 1
lyophilized Standard
Biotinylated detection antibody (50x) 100 ul
Avidin-Biotin-Peroxidase Complex (30x) 400 µl
Sample diluent 30 ml
Antibody diluent 12 ml
Avidin-Biotin-Peroxidase Diluent 12 ml
Wash Buffer (25x) 20 ml
Color Developing Reagent (TMB) 10 ml
Stop Solution 10 ml
Plate Sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse SAP

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	3608	12778	47613	3726	13449	43062
Standard deviation	144.32	715.56	2809.16	176.12	981.77	2626.78
CV (%)	4%	5.6%	5.9%	4.7%	7.3%	6.1%

Background

Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	3608	4012	4067	4138	3956	205.96	5.2%
Sample 2	12778	11342	12753	12065	12234	589.38	4.8%
Sample 3	47613	50001	47706	49348	48667	1034.13	2.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20219
Gene Name	Apcs
UniProt ID	P12246
Protein Name	Serum amyloid P-component
Sequence	Belongs to the pentaxin family.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000200	FEK1208	96 wells/kit	$499		Add to Cart

Mouse SAP/PTX2/APCS PicoKine® Quick ELISA Kit

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