Mouse Resistin ELISA Kit PicoKine®

Mfr.No.

EK0582

Sensitivity

<2 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.006	0.122	0.245	0.441	0.743	1.101	1.748	2.241

Description

Mouse Resistin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Retn in cell culture supeatants, serum and plasma (heparin). . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Resistin ;Resistin, isoform CRA_a ;Retn ;Xcp4 ;mCG_13014 ;

Molecular Weight

12492 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S21-S114

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Resistin

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	77	245	929	80	252	861
Standard deviation	6.2%2	13.23	57.59	7.28	17.64	69.74
CV (%)	7.7%	5.4%	6.2%	9.1%	7%	8.1%

Background

Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance. Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.Resistin is overexpressed in mouse adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.The standard product used in this kit is mouse Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	77	78	88	84	81	4.49	5.5%
Sample 2	245	263	267	252	256	8.72	3.4%
Sample 3	929	803	900	906	884	48.28	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	57264
Gene Name	RETN
UniProt ID	Q5BMX4
Protein Name	Resistin

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Diabetes-associated,Energy Metabolism,Energy Transfer Pathways,Growth Factors/Hormones,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000532	EK0582	96 wells/kit	$499		Add to Cart

Mouse Resistin ELISA Kit PicoKine®

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