Mouse Renin 1/Ren1 ELISA Kit PicoKine®

Mfr.No.

EK1250

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.023	0.096	0.195	0.235	0.379	0.578	1.061	1.834

Description

Mouse Renin 1/Ren1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ren1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Renin-1;3.4.23.15;Angiotensinogenase;Kidney renin;Ren1;Ren, Ren-1;

Molecular Weight

44343 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S72-R402

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Renin-1

Subcellular Localization

Secreted . Membrane . Associated to membranes via binding to ATP6AP2. .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	158	449	1246	147	482	1262
Standard deviation	10.74	28.73	64.79	11.9	34.22	70.67
CV (%)	6.8%	6.4%	5.2%	8.1%	7.1%	5.6%

Protein Function

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Background

Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	158	161	142	150	152	7.39	4.8%
Sample 2	449	465	437	476	456	14.9	3.2%
Sample 3	1246	1215	1179	1158	1199	33.7	2.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	19701
Gene Name	REN
UniProt ID	P06281
Protein Name	Renin-1
Tissue Specificity	Kidney.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000851	EK1250	96 wells/kit	$499		Add to Cart

Mouse Renin 1/Ren1 ELISA Kit PicoKine®

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