Mouse Renin 1/Ren1 ELISA Kit PicoKine®

Mfr.No.

EK1250

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.023	0.096	0.195	0.235	0.379	0.578	1.061	1.834

Description

Mouse Renin 1/Ren1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ren1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Renin-1;3.4.23.15;Angiotensinogenase;Kidney renin;Ren1;Ren, Ren-1;

Molecular Weight

44343 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S72-R402

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Renin-1

Subcellular Localization

Secreted . Membrane . Associated to membranes via binding to ATP6AP2. .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	158	449	1246	147	482	1262
Standard deviation	10.74	28.73	64.79	11.9	34.22	70.67
CV (%)	6.8%	6.4%	5.2%	8.1%	7.1%	5.6%

Protein Function

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Background

Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	158	161	142	150	152	7.39	4.8%
Sample 2	449	465	437	476	456	14.9	3.2%
Sample 3	1246	1215	1179	1158	1199	33.7	2.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	19701
Antigen	1/Ren1
Gene Name	REN
UniProt ID	P06281
Protein Name	Renin-1
Tissue Specificity	Kidney.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000851	EK1250	96 wells/kit	$499		Add to Cart

Mouse Renin 1/Ren1 ELISA Kit PicoKine®

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