Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine®

Mfr.No.

EK0832

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.090	0.141	0.192	0.300	0.521	0.939	1.677	2.337

Description

Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Rbp4 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Retinol-binding protein 4 ;Rbp4 ;

Molecular Weight

28405 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-L201

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse RBP4

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	418	2343	8435	401	2576	7635
Standard deviation	23.4	105.43	649.49	28.07	159.71	633.7
CV (%)	4.5%	4.5%	7.7%	7%	6.2%	8.3%

Background

Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that it is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard product used in this kit are recombinant mouse B7-1/CD80, D37K245, consisting of dimer acids with two single straned. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	418	421	430	389	414	15.37	3.7%
Sample 2	2343	2347	2512	2667	2467	133.97	5.4%
Sample 3	8435	8668	9897	9494	9123	595.2	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	19662
Gene Name	RBP4
UniProt ID	H7BWY6
Protein Name	Retinol-binding protein 4
Sequence	Belongs to the calycin superfamily. Lipocalin family.

Applications	ELISA
Research Area	Atherosclerosis,Cancer,Cardiovascular,Cofactors, Vitamins/Minerals,Diabetes,Diabetes-associated,Heart Disease,Metabolism,Pathways and Processes,Serum Proteins,Signal Transduction,Vitamins/Minerals

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000644	EK0832	96 wells/kit	$499		Add to Cart

Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine®

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