Mouse/Rat DCC ELISA Kit PicoKine®

Mfr.No.

EK2184

Sensitivity

<10 pg/ml

Detection Range

23.4 pg/ml - 1,500 pg/ml

Reactivity

Mouse, Rat

Sample Volume

Concentration(pg/ml)	0	23.4	46.8	93.7	187.5	375	750	1,500
O.D.	0.101	0.182	0.332	0.452	0.956	1.135	1.525	2.112

Description

Mouse/Rat DCC ELISA Kit PicoKine® (96 Tests). Quantitate Mouse/Rat Dcc in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F32-N1097

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Mouse/Rat DCC

Sample Type

Cell culture supeatants, Serum, Plasma

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	13	98	265	19	103	282
Standard deviation	0.81	6.17	15.64	0.91	5.25	15.51
CV(%)	6.2%	6.3%	5.9%	4.8%	5.1%	5.5%

Background

Netrin receptor DCC, also known as DCC, or colorectal cancer suppressor is a protein which in humans is encoded by the DCC gene. Predicted to enable identical protein binding activity; netrin receptor activity; and transcription coactivator activity. Involved in postsynaptic modulation of chemical synaptic transmission. Acts upstream of or within generation of neurons. Located in axon. Is integral component of postsynaptic density membrane. Is active in Schaffer collateral - CA1 synapse. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; lung; and retina. Human ortholog(s) of this gene implicated in colorectal cancer; congenital mirror movement disorder; esophageal cancer; and esophagus squamous cell carcinoma. Orthologous to human DCC (DCC netrin 1 receptor).

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	13	15	18	22	17	1.17	6.9%
Sample 2	98	102	113	97	103	6.66	6.5%
Sample 3	265	278	296	282	280	18.5	6.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	13176
Gene Name	Dcc
UniProt ID	P70211, Q63155

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001729	EK2184	96 wells/kit	$499		Add to Cart

Mouse/Rat DCC ELISA Kit PicoKine®

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