Mouse Pyruvate Dehydrogenase Protein X Component - Mitochondrial

Mfr.No.

E2416Mo

Alternative Names

AI481367;Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex;E3bp;Lipoyl-containing pyruvate dehydrogenase complex component X;PDHX;Pdx;Pdx1;Pyruvate dehydrogenase protein X component, mitochondrial

Reactivity

Mouse

Detection Range

15-3000ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

53999

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.33ng/L

Background

Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex (By similarity).

Gene ID	27402
UniProt ID	Q8BKZ9
Antigen	PDHX

Research Area

Signal Transduction

Protocol

These standard curves of E2416Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.333	2.336	2.295
	2.34
800ng/L	1.704	1.701	1.66
	1.698
400ng/L	1.165	1.122	1.08
	1.078
200ng/L	0.654	0.647	0.606
	0.641
100ng/L	0.431	0.423	0.382
	0.415
0ng/L	0.052	0.041	0
	0.031

Precision

We measured random samples of E2416Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	767.13	33.58	4.38
Intra-Assay	2	18	234.82	12.15	5.18
Intra-Assay	3	18	180.15	13.21	7.33

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110227	E2416Mo	48T	$320		Add to Cart
BHE12110227	E2416Mo	96T	$458		Add to Cart

Mouse Pyruvate Dehydrogenase Protein X Component - Mitochondrial - PDHX ELISA Kit

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