Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

Mouse Pulmonary surfactant-associated protein A,SP-A ELISA Kit

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Mfr.No.

CSB-E08685m

Alternative Names

surfactant protein A1, AC068139.6, MGC133365, PSAP, PSPA, SFTP1, SFTPA1, pulmonary surfactant apoprotein, surfactant, pulmonary-associated protein A1, surfactant, pulmonary-associated protein A1B

Reactivity

Mouse

Detection Range

3.12 pg/mL-200 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5000	Average %	86
	Range %	80-94
1:10000	Average %	97
	Range %	91-105
1:20000	Average %	95
	Range %	91-102
1:40000	Average %	94
	Range %	86-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.753	2.769	2.761	2.641
150	2.404	2.428	2.416	2.296
75	1.905	1.911	1.908	1.788
37.5	1.281	1.293	1.287	1.167
18.75	0.726	0.729	0.728	0.608
9.37	0.465	0.472	0.469	0.349
4.68	0.288	0.294	0.291	0.171
0	0.121	0.119	0.120	?

Species

Mus musculus

Sensitivity

0.78 pg/mL

Limit Of Detection

450 nm

UniProt ID	P35242
Antigen	surfactant protein A1

Applications

ELISA

Research Area

SFTPA1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506581	CSB-E08685m	96T	$695	Add to Cart
BHE10506581	CSB-E08685m	5x96T	$3336	Add to Cart
BHE10506581	CSB-E08685m	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.